Index of /wp-content/uploads/sites/27/2014/10

• Parent Directory
• Project-SCALE_Mission-statement.pdf
• SASoM-EQUIP-001-Autoclave-Timo.pdf
• SASoM-EQUIP-002-Aspirator-Pump-Dymax-5.pdf
• SASoM-EQUIP-003-Multipette-Eppendorf.pdf
• SASoM-EQUIP-004-Dri-Block-heaters-Stuart.pdf
• SASoM-EQUIP-005-Bag-Sealer-Hulme-Martin-.pdf
• SASoM-EQUIP-006-Gas-cylinders-for-hypoxic-work-station.pdf
• SASoM-EQUIP-007-Transblot-Apparatus-Biorad.pdf
• SASoM-EQUIP-008-mini-Protean-II-Biorad.pdf
• SASoM-EQUIP-009-H35-Hypoxystation-Don-Whitley-1.pdf
• SASoM-METHOD-002-Preparation-of-media-with-10FCS-for-Cell-Culture.pdf
• SASoM-METHOD-003-Preparation-of-media-with-5-DCC-FCS-for-Cell-Culture.pdf
• SASoM-METHOD-004-Dextran-coated-charcoal-treatment-of-FCS-for-Cell-Culture.pdf
• SASoM-METHOD-005-Preparation-of-Heat-Inactivated-FCS-for-Cell-Culture.pdf
• SASoM-METHOD-006-Preparation-of-protein-lysates-from-adherent-cells.pdf
• SASoM-METHOD-007-Preparation-and-Aliquoting-of-Supplements-for-Cell-Culture.pdf
• SASoM-METHOD-008-Use-of-Trypsin-for-passaging-cells-in-Cell-Culture.pdf
• SASoM-METHOD-009-Generating-and-working-with-viral-particles-at-Biosafety-Level-2.pdf
• SASoM-METHOD-010-Thawing-and-Freezing-of-Cells-for-Cell-Culture.pdf
• SASoM-METHOD-011-Preparation-of-Freeze-mixture-for-Cell-Culture.pdf
• SASoM-METHOD-012-Preparation-of-Heregulin-B-for-Cell-Culture.pdf
• SASoM-METHOD-013-Fast-Green-Stain.pdf
• SASoM-METHOD-014-Preparation-of-Sterile-PBS-for-Cell-Culture.pdf
• SASoM-METHOD-015-Preparation-of-Tamoxifen-stock.pdf
• SASoM-METHOD-016-Preparation-of-B-Estradiol-stock.pdf
• SASoM-METHOD-017-Preparation-of-Agarose-Embedded-Cell-Pellets-1.pdf
• SASoM-METHOD-018-Flow-Cytometric-DNA-Analysis.pdf
• SASoM-METHOD-019-Preparation-of-Type-1-Collagen.pdf
• SASoM-METHOD-020-Preparation-of-Collagen-gels-for-Spheroid-Invasion-Assays.pdf
• SASoM-METHOD-021-Collagen-Gel-Invasion-Assay.pdf
• SASoM-METHOD-022-Protein-Extraction-from-Human-Tissue-and-Primary-Xenografts.pdf
• SASoM-METHOD-023-Protein-Determination-by-the-Bicinchoninic-Acid-BCA-Assay.pdf
• SASoM-METHOD-024-Dewaxing-and-Rehydrating-Paraffin-Sections.pdf
• SASoM-METHOD-025-Immunohistochemistry.pdf
• SASoM-METHOD-026-Microvigene-software-Analysis.pdf
• SASoM-METHOD-027-MicroVigene-software-Platemaps.pdf
• SASoM-METHOD-028-MicroVigene-software-Template.pdf
• SASoM-METHOD-029-Optimising-Antibodies-using-immunofluorescent-staining.pdf
• SASoM-METHOD-030-In-house-quality-control-slide-for-IF.pdf
• SASoM-METHOD-031-Immunofluorescence-with-Epithelial-Mask-Visualisation-using-MOUSE-and-RABBIT-Primary-Antibodies.pdf
• SASoM-METHOD-032-Immunofluorescence-using-a-RABBIT-Primary-Antibody.pdf
• SASoM-METHOD-033-Western-Blot-Polyacrylamide-Gel-Electrophoresis.pdf
• SASoM-METHOD-034-Western-Blot-Antibody-Detection-Using-Licor-Odyssey-Scanner.pdf
• SASoM-METHOD-035-SRB-Growth-Assay.pdf
• SASoM-METHOD-036-Alamar-blue-Growth-Assay-1.pdf
• SASoM-METHOD-037-Prep-of-2-fold-dilution-for-RPPA.pdf
• SASoM-METHOD-038-RPPA-Detection-on-1-Pad-Fastslide.pdf
• SASoM-METHOD-039-RPPA-Detection-on-2-Pad-Fastslide.pdf
• SASoM-METHOD-040-RPPA-Detection-on-16-Pad-Fastslide.pdf
• SASoM-METHOD-041-Haematoxylin-and-Eosin-HE-Staining.pdf
• SASoM-METHOD-043-Exosome-Isolation-Using-Centrifugation.pdf
• SASoM-METHOD-044-Exosome-Quantification-Using-NanoSight-Tracking-Analysis.pdf
• SASoM-METHOD-045-Exosome-Red-Fluorescent-Labelling.pdf
• SASoM-METHOD-046-shRNA-cloning.pdf
• SASoM-METHOD-047-siRNA-transfection.pdf
• SASoM-METHOD-048-Dundee-sequencing.pdf
• SASoM-METHOD-049-Transwell-Boyden-Chamber-Migration-Assay.pdf
• SASoM-METHOD-050-Biorad-mini-western-blotting.pdf
• SASoM-METHOD-051-Amplified-Immunofluorescence.pdf
• SASoM-METHOD-052-Agarose-Gels.pdf
• SASoM-METHOD-053-DNA-Maxi-Prep.pdf
• SASoM-METHOD-054-Use-of-CalcuSyn-software.pdf
• SASoM-METHOD-055-Mycoplasma-Test.pdf
• SASoM-METHOD-056-Cell-Tracker.pdf
• SASoM-METHOD-057-8-Chamber-Slides.pdf
• SASoM-METHOD-058-Establishment-of-3D-Spinner-Spheroids.pdf
• SASoM-METHOD-059-Establishment-of-3D-96-well-Ultra-Low-Attachment-Spheroids.pdf
• SASoM-METHOD-060-Fixation-of-3D-Spheroids.pdf
• SASoM-METHOD-061-Labelling-cells-with-Hypoxyprobe.pdf
• SASoM-METHOD-062-Agarose-Embedding-of-Spheroids.pdf
• SASoM-METHOD-063-Preparation-of-2-fold-Dilutions-for-16-pad-RPPA-Antibody-Optimisation.pdf
• SASoM-METHOD-064-RNAscope-2.0-DAB-Protocol-for-Perfusion-fixed-Cryosectioned-Tissue.pdf
• SASoM-METHOD-065-Calculating-IC50-values-using-GraphPad-Prism-6-software.pdf
• SASoM-METHOD-066-Preparation-of-5X-Loading-Buffer.pdf
• SASoM-METHOD-067-Preparation-of-Saturated-Bromophenol-Blue.pdf
• SASoM-METHOD-068-Preparation-of-4X-RPPA-Denaturating-Buffer.pdf
• SASoM-METHOD-069-Preparation-of-protein-from-FFPE-Paraffin-blocks.pdf
• SASoM-METHOD-070-Protein-Determination-by-the-Biorad-RC-DC-Protein-Assay.pdf
• SASoM-METHOD-071-Preparation-of-2-fold-Sample-Dilutions-from-FFPE-for-RPPA-Spotting.pdf
• SASoM-METHOD-072-Duplex-Immunofluorescence-with-Amplified-and-Non-Amplified-MOUSE-and-RABBIT-Primary-Antibodies.pdf
• SASoM-METHOD-073-Lentivirus-production-from-293T-cells-1.pdf
• SASoM-METHOD-074-Duplex-Immunofluorescence-using-MOUSE-and-RABBIT-Primary-Antibodies-with-DM5500-B-Microscope.pdf
• SASoM-METHOD-075-GeCKO-lentiCRISPRv2-library-DNA-amplification-in-Lucigen-bacteria-2.pdf
• SASoM-METHOD-076-Amplified-Immunofluorescence-using-MOUSE-and-RABBIT-Primary-Antibodies.pdf
• SASoM-METHOD-077-GeCKO-genomic-DNA-purification-Qiagen-Midi-kit.pdf
• SASoM-METHOD-078-Transduction-of-GECKO-Library-in-MiaPaCa2-cells.pdf
• SASoM-METHOD-079-DNA-quantification-using-QubiT-fluorometer-2.0-for-GeCKO-library.pdf
• SASoM-METHOD-080-PCR-for-GeCKOv2-library-preparation-for-Next-generation-sequencing.pdf
• SASoM-METHOD-081-sgRNA-Target-Guide-Sequence-Cloning-into-pLentiCRISPR-v2-1.pdf
• SASoM-METHOD-082-AMPure-PCR-purification-for-GeCKOv2-NGS.pdf
• SASoM-METHOD-083-Quality-control-of-GeCKOv2-for-NGS-using-Agilent-Bioanalyzer-system.pdf
• SASoM-METHOD-084-Colony-Formation-Assay-with-Celigo-Visualisation.pdf
• SASoM-METHOD-085-MCM2-IHC-using-DakoLink48-003.pdf
• SASoM-METHOD-086-MCM2-and-Rabbit-pan-cytokeratin-IF-with-Hoescht-counterstain-using-Dako-Link48.pdf
• SASoM-METHOD-087-MCM2-and-Rabbit-pan-cytokeratin-IF-with-Haematoxylin-counterstain-using-Dako-Link48.pdf
• SASoM-METHOD-088-Preparation-of-clinical-sample-slides-using-Surepath-LBC-System.pdf
• SASoM-METHOD-089-Multiplexing-Immunofluorescence-for-four-primary-proteins.pdf
• SASoM-METHOD-090-Immunofluorescence-for-two-same-species-antibodies-using-tyramide-signal-amplification-TSA.pdf
• SASoM-METHOD-091-Multiplexing-Immunofluorescence-for-three-target-proteins.pdf
• SASoM-METHOD-092-Fixing-cells-in-urine-for-Hologic-slide-preparation.pdf
• SASoM-METHOD-093-Preparation-of-RNA-using-the-Qiagen-RNeasy-kit.pdf
• SASoM-METHOD-094-2D-Cell-Viability-Assay-using-Calcein-AM-PI-and-Hoescht-33342.pdf
• SASoM-METHOD-095-3D-Cell-Viability-Assay-using-Calcein-AM-PI-and-Hoescht-33342.pdf
• SASoM-METHOD-096-Preparation-and-Fixation-of-Cell-Culture-Cytospin-preparations.pdf
• SASoM-METHOD-097-Preparation-and-Chicken-Red-Blood-Cells.pdf
• SASoM-METHOD-098-Preparation-of-samples-for-Annexin-V-Assay.pdf
• SASoM-METHOD-099-Preparation-of-Samples-for-Transmission-Electron-Microscopy.pdf
• SASoM-METHOD-100-Phalloidin-Staining-of-cells.pdf
• SASoM-METHOD-101-BrdU-incorporation-for-Flow-Cytometric-Analysis.pdf
• SASoM-METHOD-102-Preparation-of-protein-lysates-from-adherent-cells-using-RIPA-buffer.pdf
• SASoM-METHOD-103-Culture-fixation-and-immunocytochemistry-of-cells-on-coverslips-for-immunofluorescent-imaging.pdf
• SASoM-METHOD-105-In-Cell-Western-with-Li-COR-Secondary-Antibodies.pdf
• SASoM-METHOD-106-F-actin-staining-using-Phalloidin-iFluor-488.pdf
• SASoM-METHOD-107-Oil-Red-O-staining-of-cultured-cells.pdf
• SASoM-METHOD-108-Preparation-of-protein-lysates-from-adherent-cells-using-NEB-Lysis-Buffer.pdf
• SASoM-METHOD-109-Neutral-COMET-Assay.pdf
• SASoM-METHOD-110-BrdU-Incorporation-of-cells-grown-in-Chamber-Slides-or-on-Cover-Slips.pdf
• SASoM-METHOD-111-MTT-Assay.pdf
• SASoM-METHOD-112-Preparation-of-Cell-Lines-for-HPLC-analysis-of-GSSG-and-GSH.pdf
• SASoM-METHOD-113-Revert-700-Total-Protein-Stain-Normalization.pdf
• SASoM-METHOD-114-rtPCR-using-Qiagen-QuantiTect-reverse-transcription-kit-PROTOCOL-MISSING.pdf
• SASoM-METHOD-115-RNA-Isolation-using-TRI-Reagent.pdf
• SASoM-METHOD-116-PolyA-RNA-isolation-using-NEXTFLEX-PolyA-Beads.pdf
• SASoM-METHOD-117-Martius-Scarlet-Blue-Staining.pdf
• SASoM-METHOD-118-Periodic-acid-Schiff-Staining.pdf
• SASoM-METHOD-119-Massons-Staining.pdf
• SASoM-METHOD-120-Mallorys-Staining.pdf
• SASoM-METHOD-121-Carstairs-Picric-Acid-Staining.pdf
• SASoM-METHOD-122-Millers-Picric-Acid-Staining.pdf
• SASoM-METHOD-123-Picro-Sirius-Red-Picric-Acid.pdf
• SASoM-METHOD-124-Analysis-of-NUC-1031-NUC-3373-and-NUC-7738-by-uHPLC.pdf
• SASoM-METHOD-125-Analysis-of-ATP-ADP-AMP-and-other-nucleotides-by-uHPLC..pdf
• SASoM-METHOD-126-Analysis-of-Glutathione-by-uHPLC.pdf
• SASoM-METHOD-127-Cell-Cycle-Progression-by-Flow-Cytometry.pdf
• SASoM-METHOD-128-Antibody-Detection-by-Flow-Cytometry.pdf
• SASoM-METHOD-129-DNA-extraction-using-the-QIAmp-DNA-Kit.pdf
• SASoM-METHOD-130-Congo-Red-Staining.pdf
• SASoM-METHOD-131-Perls-Prussian-Blue-Staining.pdf
• SASoM-METHOD-132-Total-Protein-Stain-using-Fastgreen.pdf
• SASoM-METHOD-133-IHC-and-IF-using-Leica-Bond-RX.pdf
• SASoM-METHOD-134-Preparation-of-Media-for-Organoid-Cultures.pdf
• SASoM-METHOD-135-Culture-of-PBMCs.pdf
• SASoM-METHOD-136-Preparation-of-metabolites-for-MS-analysis.pdf
• SASoM-METHOD-137-Analysis-of-Adenine-Adenosine-and-Cordycepin-levels-by-uHPLC.pdf
• SASoM-METHOD-137-Preparation-of-metabolites-for-MS-analysis.pdf
• SASoM-METHOD-138-Monitoring-cell-division-using-the-CFSE-Cell-Division-Tracker-Kit.pdf
• SASoM-METHOD-139-Use-of-GolgiStop-Protein-Transport-Inhibitor.pdf
• SASoM-METHOD-140-DNA-extraction-using-the-QIAmp-DNA-kit.pdf
• SASoM-METHOD-141-DNA-RNA-hydrolysis-for-analysis-by-mass-spectrometry.pdf
• SASoM-METHOD-142-Generation-of-dendritic-cells-from-PBMCs-for-in-vitro-experiments.pdf
• SASoM-METHOD-143-Use-of-the-checkpoint-inhibitor-Nivolumab-in-cell-culture-experiments.pdf
• SASoM-METHOD-144-Sectioning-FFPE-blocks-using-Leica-RM2255-microtome.pdf
• SASoM-METHOD-145-Fluorescence-In-situ-hybridisation-FISH.pdf
• SASoM-METHOD-146-In-Hwa-Embedding-of-Formalin-Fixed-Paraffin-Embedded-FFPE-tissue.pdf
• SASoM-METHOD-147-Tissue-microarray-construction-using-Minicore-TMA.pdf
• SASoM-METHOD-148-RNA-in-situ-hybridisation.pdf
• SCALE-poster.pdf
• SaSoM-METHOD-042-Exosome-Isolation-Using-Antibody-Coated-Beads.pdf
• organisation_HR_Sickness-Absence.pdf
• organisation_HR_cpd-and-disc-travel-form.xlsx
• organisation_HR_cpd-and-disc-travel.pdf
• organisation_HR_departure-of-staff.pdf
• organisation_finance_application-form.pdf
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• students_UG-allocation_Manchester-visit.pdf